Calibration Standards for Flow Cytometry Applications
Calibration particles are essential for the routine optimization and quality assurance of flow cytometers. We are offering size and fluorescence signal standards, which can ensure accurate signal detection, consistent data acquisition, and reliable instrument performance across experiments.
Size Calibration Standards for Flow Cytometry
Ensure precision and consistency in your flow cytometry experiments with our high-quality calibration beads, which are made from highly monodisperse polystyrene microspheres with low coefficient of variation (CV < 1,2 %). These size calibration standards can be used to validate the forward scatter (FSC) and side scatter (SSC) parameters, which are crucial for assessing cell size and granularity.
Key Features:
Size distribution:
CV < 1,2 %
Characterization:
compatible with most benchtop and high-throughput cytometers
Fluorescence Calibration Standards:
Our fluorescence calibration particles ensure reliable evaluation of your detector’s sensitivity. The fluorescence standards consist of 3 µm polystyrene particles with embedded fluorescent dye with defined Mean Fluorescence Intensity (MFI) values. This allows you to reliably and reproducibly adjust the voltage optimization of your photomultiplier tubes (PMTs) in your flow cytometer.
Thus you can ensure both dim and bright signals are captured without saturation or loss. These Single-color beads simplify spectral overlap correction in multicolor panels.
Key Features:
Size distribution:
CV < 2%
Fluorecence channels
FITC, APC,
Fluorescence Intesity
Low, Mid, High,
Excitation/Emission profiles:
Matched to standard laser lines (e.g., 488 nm, 632 nm)
It is also possible to obtain particles with graded fluorescence intensities, which contributes to the validation of the linear response of detectors and amplifiers over a dynamic range.
Contact us for customized solutions – we will be happy to advise you!
